Fine scale differences within the vagal neural crest for enteric nervous system formation

JE Simkin; D Zhang; LA Stamp; DF Newgreen

Journal article

Fine scale differences within the vagal neural crest for enteric nervous system formation

JE Simkin, D Zhang, LA Stamp, DF Newgreen

Developmental Biology | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2019

DOI: 10.1016/j.ydbio.2018.11.007

Abstract

The enteric nervous system is mostly derived from vagal neural crest (NC) cells adjacent to somites (s)1–7. We used in ovo focal fluorescent vital dyes and focal electroporation of fluorophore-encoding plasmids in quail embryos to investigate NC cell migration to the foregut initially and later throughout the entire gut. NC cells of different somite-level origins were largely separate until reaching the foregut at about QE2.5, when all routes converged. By QE3.5, NC cells of different somite-levels became mixed, although s1-s2 NC cells were mainly confined to rostral foregut. Mid-vagal NC-derived cells (s3 and s4 level) arrived earliest at the foregut, and occurred in greatest number. By QE6..

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University of Melbourne Researchers

Lincon Stamp Author

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Grants

Awarded by Eunice Kennedy Shriver National Institute of Child Health and Human Development

Funding Acknowledgements

This work was supported by NHMRC grants (DFN and DZ: 607379 and 1069757, and LS: 1079234). LS is also a recipient of an Australian Research Council DECRA Fellowship (DE180100261). MCRI facilities are supported by the Victorian Government's Operational Infrastructure Support Program. Dr. Craig Smith, MCRI, supplied the rabbit SoxE antibody. QCPN and E/C8 antibodies were supplied by the Developmental Studies Hybridoma Bank (http://dshb.biology.uiowa.edu/) under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, IA 52242. Dr. Yoshiko Takahashi provided the pT2K-CAGGS-GFP and pCAGGS-T2TP expression constructs. The late Dr. Catherine Krull donated the pMes GFP construct. Dr Peter Farlie, MCRI, assisted with preparation of the dsRedExpress2 construct.